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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Pancreas. 2012 Aug;41(6):962–969. doi: 10.1097/MPA.0b013e31823d0160

FIGURE 1.

FIGURE 1

Temporal expression of Pdx1, Ptf1a, Neurog3, Hes1, Sox9 and Rfx6 mRNA by quantitative PCR. Q-RTPCR was performed on cDNA synthesized from fetal pancreas and adult CD1 islets using a 5' nuclease assay and FAM ® dye labeled Taqman MGB probes with two PCR primers. Endogenous gapdh was used for normalization. Data (mean ± SE) is expressed relative to a control sample (E12.5). For statistical analysis one-way analysis of variance (ANOVA), probability value (p < 0.05) was considered significant, followed by Student's T- test (p<0.05).