Identification of 4 novel genes during murine pancreatic development. Expression of Cbs, Gfi1, Meis1, and Aldh18a1 mRNA by quantitative PCR. Q-RTPCR was performed on cDNA synthesized from fetal pancreas and adult CD1 islets using a 5' nuclease assay and FAM ® dye labeled Taqman MGB probes with two PCR primers. Endogenous GAPDH was used for normalization. Data (mean ± SE) is expressed relative to a control sample (E12.5). For statistical analysis one-way analysis of variance (ANOVA), probability value (p < 0.05) was considered significant, followed by Student's T- test (p<0.05).