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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Arthritis Rheum. 2012 Aug;64(8):2482–2488. doi: 10.1002/art.34459

Figure 2. Cytokine production in response to CII259-273 variants.

Figure 2

(A). Expression of intracellular IL-17, IFNγ and IL-2 in CD4+ CD154+ T cells stimulated with the superantigen SEB and further diluted 10 times in a two-fold serial dilution. The threshold of detection was set to <0.01% (B). T cell responses towards the four variants of CII259-273; CII-K, CII-Gal264, CII-Gal270 and CII-Gal264/270 was monitored by multicolor flow cytometry in 10 RA patients following short term in vitro stimulation of PBMC. The expression levels (%) of IL-17, IFNγ and IL-2 after subtracting background levels are displayed. (C). IL-17, IFNγ and IL-2 expression following stimulation with the CII259-273 variants in 10 HLA-DR*0401 matched healthy control samples are displayed. (D). Combined analysis of the sum of cytokines produced in response to the CII peptide variants. *P < 0.05