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. Author manuscript; available in PMC: 2013 Jul 11.
Published in final edited form as: Chem Rev. 2012 Mar 16;112(7):4221–4245. doi: 10.1021/cr100351g

Figure 21.

Figure 21

Polyacrylamide gel data showing multiple additions of pyrene nucleoside triphosphate at the end of a DNA primer. Lane 1: 10 bp size marker, lane 2: primer only, lanes 3-11: TdT reaction with increasing concentrations (2.5–640 μm) of α -dPTP, lanes 12-20: TdT reaction with increasing concentrations (2.5-640 μm) of β-dPTP. TdT was capable of incorporating up to three α-dPTPs or four β-dPTPs. Reprinted with permission from ref 87. Copyright 2006 Wiley-VCH Verlag GmbH & Co. KGaA.