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. 2012 Jul;40-334(7):588–598.e1. doi: 10.1016/j.exphem.2012.02.006

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© 2012 Elsevier Inc.

Open Access under CC BY 4.0 license

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Scl^Δ19/Δ19 mice are viable and have normal mature hematopoietic lineages. (A) Left panel shows schematic representation of the Scl alleles used in this study: Scl^WT/WT and Scl^Δ19/Δ19 locus with the deletion of a 2.4-kb region containing both the +18 (light green bar) and +19 enhancers (dark green bar). Black triangle represents loxP site remaining in the genome after Cre recombination. Scl exons are depicted in red and the Map17 exons in blue. Right panel shows PCR genotyping analysis of WT (Scl^WT/WT), homozygous (Scl^Δ19/Δ19), and heterozygous (Scl^Δ19/WT) knockout alleles. In the first lane is 1-kb DNA marker. (B) Analysis of total cellularity from the BM and spleen in Scl^WT/WT and Scl^Δ19/Δ19 adult mice. (C) Percentage of granulocytes (Gr1⁺Mac1⁺), megakaryocytes (CD41⁺), and erythrocytes (Ter119⁺) cells in BM and T cells (CD4⁺, CD8⁺) and B cells (B220⁺) in spleen of Scl^WT/WT and Scl^Δ19/Δ19 mice. (D) Scl expression in mature blood lineages of the BM and spleen in Scl^Δ19/Δ19 mice. Data are presented as relative expression to Scl^WT/WT. Erythroid cells were sorted using Ter119 antibody, megakaryocytes using CD41, macrophages using Mac-1 and T cells from spleen using CD4. (E) Mast cells are normal in Scl^Δ19/Δ19 mice. Left panel shows peritoneal cells stained with Toludine blue and Metachromatic staining of mast cells. Right panel shows quantitative analysis of mast cells (cKit⁺ Sca1⁺) from peritoneal wash in Scl^WT/WT and Scl^Δ19/Δ19 mice.

Scl^Δ19/Δ19 mice are viable and have normal mature hematopoietic lineages. (A) Left panel shows schematic representation of the Scl alleles used in this study: Scl^WT/WT and Scl^Δ19/Δ19 locus with the deletion of a 2.4-kb region containing both the +18 (light green bar) and +19 enhancers (dark green bar). Black triangle represents loxP site remaining in the genome after Cre recombination. Scl exons are depicted in red and the Map17 exons in blue. Right panel shows PCR genotyping analysis of WT (Scl^WT/WT), homozygous (Scl^Δ19/Δ19), and heterozygous (Scl^Δ19/WT) knockout alleles. In the first lane is 1-kb DNA marker. (B) Analysis of total cellularity from the BM and spleen in Scl^WT/WT and Scl^Δ19/Δ19 adult mice. (C) Percentage of granulocytes (Gr1⁺Mac1⁺), megakaryocytes (CD41⁺), and erythrocytes (Ter119⁺) cells in BM and T cells (CD4⁺, CD8⁺) and B cells (B220⁺) in spleen of Scl^WT/WT and Scl^Δ19/Δ19 mice. (D) Scl expression in mature blood lineages of the BM and spleen in Scl^Δ19/Δ19 mice. Data are presented as relative expression to Scl^WT/WT. Erythroid cells were sorted using Ter119 antibody, megakaryocytes using CD41, macrophages using Mac-1 and T cells from spleen using CD4. (E) Mast cells are normal in Scl^Δ19/Δ19 mice. Left panel shows peritoneal cells stained with Toludine blue and Metachromatic staining of mast cells. Right panel shows quantitative analysis of mast cells (cKit⁺ Sca1⁺) from peritoneal wash in Scl^WT/WT and Scl^Δ19/Δ19 mice.