Figure 2.

Phenotypic analysis of progenitor cells in adult BM of WT and Scl^Δ19/Δ19 mice. (A) Scl^Δ19/Δ19 animals have an increased number of Lin⁻Sca1⁺Kit⁺ cells compared with the Scl^WT/WT. Histogram shows percentage of Sca1⁺Kit⁺ cells within the Lin⁻ BM cells of Scl^WT/WT and Scl^Δ19/Δ19 mice. (B) No significant difference is observed in the number of long-term CD34⁻ stem cells in the two genotypes; however, there is a 2-fold increase in short-term CD34⁺ stem cells in Scl^Δ19/Δ19 mice. Histogram show percentage of CD34⁺ and CD34⁻ within the LSK population in BM. (C) Analysis of BM progenitor population reveals a normal population of granulocyte-macrophage progenitors (GMPs) and common myeloid progenitors but an increase in the megakaryocytic erythroid progenitor (MEP) population in Scl^Δ19/Δ19. (D) No difference was observed in the number of common lymphoid progenitors (CLPs) between WT and Scl^Δ19/Δ19 animals. (E) Expression level of Scl in short-term (LSK CD34⁺) and long-term (LSK CD34⁻) stem cells. (F) Cell cycle fluorescence-activated cell sorting analysis on sorted LSK population stained for the Ki-67 and propidium iodide (PI) in Scl^WT/WT and Scl^Δ19/Δ19 mice. Left panel shows representative plots and right panel shows quantification results. Analysis was performed on six animals from each genotype.