Figure 3.

Enhanced long-term repopulation in Scl^Δ19/Δ19 mice. (A) Outline of transplantation assay. CD45.2 Scl^WT/WT or Scl^Δ19/Δ19 unfractionated BM was mixed with CD45.1 unfractionated BM at a ratio of 1:1. Five to six lethally irradiated CD45.1 recipients were injected with 1 × 10⁶ cells per group (Scl^Δ19/Δ19 or Scl^WT/WT donor CD45.2). (B) Percentage reconstitution (CD45.2⁺ cells) measured 4 weeks post-transplantation by fluorescence-activated cell sorting (FACS) analysis of peripheral blood with CD45.1 and CD45.2 antibodies. (C) Recipient peripheral blood FACS analysis for different lineage markers: B cells (B220), macrophages (Mac-1), and T cells (CD4). CD45.2 donor reconstitution for each lineage is expressed as a percentage. (D) Percentage reconstitution measured 12 weeks post-transplantation by FACS analysis of peripheral blood with CD45.1 and CD45.2 antibodies. Two independent transplantations are shown. (E) Recipient peripheral blood FACS analysis for different lineage markers: B cells (B220), macrophages (Mac-1), and T cells (CD4). CD45.2 donor reconstitution for each lineage is expressed as a percentage.