Figure 4.

Decreased activity at Scl promoters in Scl^Δ19/Δ19 mice. (A) The mouse Scl and neighboring Map17 gene loci. Scl exons are depicted in red and the Map17 exons in blue. Arrows indicate functionally characterized Scl regulatory elements. The number above each arrow indicates the position, in kb, of the enhancer in relation to the Scl promoter 1a. 1a- Scl Promoter 1a; 1b- Scl Promoter 1b. (B) ChIP assay for active (H3K4me3 and H3K9Ac) and repressive (H3K9me3 and H3K9me2) chromatin marks. Fold enrichment was compared with that of the IgG control. (C) qRT-PCR analysis of Scl primary transcript and mRNA expression in E14.5 fetal liver cells shows a reduction of primary transcript levels in enhancer deleted cells.