Fig. 8.

Effect of TGFβ1 on PR expression in HESC. A, Representative immunoblot of PR isoforms in HESC treated with progesterone, TGFβ1, or both for 48 h. Maximal effects were observed with progesterone (10⁻⁷ m) and TGFβ1 (5 ng/ml) for 48 h (not shown). GAPDH was used as a loading control. Fibroblasts infected with PR-A or PR-B adenovirus were used as positive controls. B and C, Data represent mean ± sem of relative signal intensities in four immunoblots conducted with samples from four different cell preps. Vehicle-treated control (CTL) cells served as baseline expression of PR-B (B) and PR-A (C). *, P < 0.01 compared with CTL (paired); **, P < 0.05 compared with progesterone (Prog).