Table 1.
Dissociation constants for binding at the high-affinity (KD1) and low-affinity (KD2) RRE RNA sites by M-Chelate-Rev species determined by AP-RNA fluorescence titration experiments.
| Complex | KD1 (nM) | KD2 (nM) |
|---|---|---|
| [DOTA]3−–Rev | 0.2 ± 0.2 | 500 ± 30 |
| [Fe-DOTA]1−–Rev | 0.36 ± 0.08 | 1120 ± 20 |
| [Co-DOTA]1−–Rev | 2.1 ± 0.2 | 2550 ± 40 |
| [Ni-DOTA]1−–Rev | 2.3 ± 0.2 | 1180 ± 20 |
| [Cu-DOTA]1−–Rev | 0.4 ± 0.1 | 488 ± 9 |
| [EDTA]3−–Rev | 0.8 ± 0.4 | 1150 ± 30 |
| [Fe-EDTA]1−–Rev | 5.6 ± 0.7 | 630 ± 10 |
| [Co-EDTA]1−–Rev | 0.5 ± 0.3 | 560 ± 10 |
| [Ni-EDTA]1−–Rev | 0.8 ± 0.3 | 520 ± 10 |
| [Cu-EDTA]1−–Rev | 1.9 ± 0.3 | 1160 ± 20 |
| [DTPA]4−–Rev | 23 ± 1 | 980 ± 20 |
| [Fe-DTPA]2−–Rev | 0.7 ± 0.8 | 430 ± 20 |
| [Co-DTPA]2−–Rev | 13.7 ± 0.7 | 1330 ± 20 |
| [Ni-DTPA]2−–Rev | 4.2 ± 0.3 | 1840 ± 20 |
| [Cu-DTPA]2−–Rev | 16 ± 2 | 1570 ± 80 |
| [NTA]2−–Rev | 25.3 ± 0.8 | 910 ± 10 |
| [Fe-NTA]0–Rev | 7.0 ± 0.4 | 950 ± 10 |
| [Co-NTA]0–Rev | 9.4 ± 0.4 | 1210 ± 10 |
| [Ni-NTA]0–Rev | 2.3 ± 0.1 | 1160 ± 10 |
| [Cu-NTA]0–Rev | 3.5 ± 0.3 | 857 ± 9 |
| [GGH]1+–Rev | 2.1 ± 0.2 | 924 ± 9 |
| [Co-GGH]0–Rev | 3.8 ± 0.6 | 360 ± 10 |
| [Ni-GGH]0–Rev | 1.3 ± 0.3 | 458 ± 8 |
| [Cu-GGH]0–Rev | 4.6 ± 0.5 | 363 ± 9 |
| [KGHK]3+–Rev | 77 ± 1 | 1020 ± 10 |
| [Co-KGHK]2+–Rev | 1.4 ± 0.4 | 300 ± 6 |
| [Ni-KGHK]2+–Rev | 3.2 ± 0.4 | 173 ± 3 |
| [Cu-KGHK]2+–Rev | 1.3 ± 0.5 | 148 ± 5 |
| Rev peptide | 1.6 ± 0.3 | 770 ± 20 |