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. Author manuscript; available in PMC: 2012 Jul 2.
Published in final edited form as: J Am Chem Soc. 2011 Jun 6;133(25):9912–9922. doi: 10.1021/ja203057z

Table 3.

Comparison of second-order rate constants for nucleic acid cleavage promoted both by several M-chelate-Rev complexes, and by other artificial catalysts.

Complex Substrate k2 (M−1 min−1) Coreagents Conditions Ref.
[Cu-NTA-Rev]9+/8+ RRE RNA 460000 ascorbate + H2O2 pH 7.4, 37 °C a
[Cu-DOTA-Rev]8+/7+ RRE RNA 250000 ascorbate + H2O2 pH 7.4, 37 °C a
[Cu-KGHK-Rev]12+/11+ RRE RNA 23000 ascorbate + H2O2 H 7.4, 37 °C a
[Cu-GGH-Rev]10+/9+ RRE RNA 12000 ascorbate + H2O2 pH 7.4, 37 °C a
[Cu-GGH-Rev]10+/9+ RRE RNA 700 ascorbate pH 7.4, 37 °C 10
[Cu-KGHK]2+/1+ tRNAPhe 6.3 ascorbate pH 7.4, 25 °C 19
[Cu-KGHK]2+/1+ dsDNA 5580 ascorbate pH 7.4, 37 °C 6
[Cu-GGH]0/1− dsDNA 2340 ascorbate pH 7.4, 37 °C 6
[Cu-neamine]4+ dsDNA 7690b none pH 7.3, 37 °C 20
[Co-cyclen](4-5)+ dsDNA 13-90b none pH 7.0, 37 °C 21
Eu3+ dsDNA 108b none pH 7.0, 37 °C 22
[Rh-(phi)2bpy’-peptide(Zn)]0 dsDNA 300 none pH 6.0, 37 °C 23
[Co-tamen]3+ dsDNA 3 none pH 7.6, 37 °C 24
[Ce2-HXTA]3+ ssDNA 840 none pH 8.0, 37 °C 25
[Ce2-HXTA]3+ dsDNA 78 none pH 8.0, 37 °C 25
(Zn)2-bis(TACN)-linker HPNP 0.348-15 none pH 7.6, 25 °C 26
(Zn)-TACN HPNP 0.126 none pH 7.6, 25 °C 26
(Zn)2(calix[4]arene) HPNP 78.8 none pH 7, 25 °C 27
LuCl3 ApA 38 none pH 7.2, 30 °C 28
a

This study.

b

Enzyme-like activity is expressed as kcat/KM. dsDNA = double stranded DNA; ssDNA = single stranded DNA; HPNP = 2-hydroxypropyl p-nitrophenyl phosphate; ApA = adenylyl(3′-5′)adenosine.