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. Author manuscript; available in PMC: 2012 Jul 2.
Published in final edited form as: Science. 2010 Nov 11;330(6008):1238–1240. doi: 10.1126/science.1195320

Figure 4.

Figure 4

Lynx1 may adjust cortical excitatory-inhibitory balance to regulate adult plasticity. (A) In WT animals (left), mature excitatory-inhibitory balance is maintained by lynx1 that limits nAChR response. In lynx1 KO mice (right), enhanced nAChR signaling may lead to excitatory-inhibitory imbalance and adult plasticity, which could be sensitive to acute restoration of inhibition with diazepam (DZ). (B) Double in situ hybridization of lynx1 (green) with GAD65 (red, upper panel) or parvalbumin (PV, lower panel) in adult V1 (left). Scale, 100µm. Quantification of overlapping pixels (right) indicates selective expression of lynx1 in a subset (40%) of GAD65-positive interneurons, most likely PV-positive cells (> 90% co-localization). (C) Focal diazepam infusion during adult MD in lynx1 KO mice abolishes ocular dominance plasticity (black, DZ: CBI = 0.67, 6 mice vs grey, vehicle: CBI = 0.54, 14 mice; *** P < 0.001, t-test). Dark circles represent cortical mini-pump infusion.