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. Author manuscript; available in PMC: 2012 Oct 13.
Published in final edited form as: Neuroscience. 2011 Jul 14;193:89–99. doi: 10.1016/j.neuroscience.2011.07.021

Fig. 2. Zinc inhibition of ASIC1b currents in ASIC1b transfected CHO cells is independent of pH activation and steady-state desensitization.

Fig. 2

(A) Original current traces showing inhibitory effects of 30 μM zinc with pretreatment on the pH-dependent activation of ASIC1b currents in CHO cells. The starting pH for all experiments was 7.4, the activating pH is indicated by bars above the trace and CHO cells were clamped at −60 mV. (B) Bar graphs showing relative amplitude of ASIC1b current inhibition by pre-applied 30 μM zinc. Each point represents the average response of eight to twelve cells. There are no significant differences between different groups in the presence of 30 μM zinc (p > 0.05, ANOVA). (C) Original current traces showing inhibitory effects of 30 μM zinc with pretreatment on steady-state desensitization of ASIC1b currents in CHO cells. Steady-state desensitization was induced by various conditioning pH values between 7.8 and 6.8 for 36 min before application of pH 5.0. CHO cells were clamped at −60 mV. (D) Bar graphs showing relative amplitude of ASIC1b current inhibition on steady-state desensitization by pre-applied 30 μM zinc at various conditioning pH values. ASIC1b current was evoked by application of pH 5.0. Each point represents the average response of five cells. There are no significant differences between each group in the presence of 30 μM zinc (p > 0.05, ANOVA). CTRL represents control.