BaCl2 partially blocks the net capsaicin currents. A. representative current in gap-free mode at holding voltage of −44 mV. The vertical spike currents, such as a, b, c and d, were caused by the I–V step voltage commands as shown in B. B. net capsaicin (300 μM) currents (a–b) in control bath. The zero current was shown as a solid horizontal bar preceding the step currents in B and C. C. net capsaicin (300 OM) currents (c–d) in the bath with 10 mM BaCl2. D. representative I–V curves of net currents in B and C. The current for each V is the 100 ms average bounded by the arrows. E. bar graph summary: the net capsaicin currents at Vh of −40 mV in the control (ctrl) and BaCl2 (10 mM) bath. The currents were normalized by the currents of ctrl. * P<0.05, n=3.