Figure 1. DISC1 variants inhibit Wnt signaling and cell proliferation.

(A) HEK293 cells were transfected with the TCF/LEF luciferase reporter together with GFP or the indicated DISC1 variant. Cells were then stimulated with either control or Wnt conditioned media (CM) for 16 hours and luciferase activity was determined. The graph represents relative Wnt-induced luciferase reporter activity. *p<0.05, NS=not significant. Error bars represent standard error of the mean (SEM). (B) P19 cells were transfected with the TCF/LEF luciferase reporter, control, or mouse DISC1 shRNA, together with the indicated hDISC1 variant and then treated as in (A). *p<0.05, NS=not significant. Error bars represent SEM. (C) N2A cells were transfected with the indicated constructs for 24 hours at which point cells were fixed and immunostained. The graph represents the percentage of transfected cells that were positive for phosphohistone H3 staining. *p<0.05, NS=not significant. Error bars represent standard SEM. (D) N2A cells were plated at equal densities and transfected with GFP, WT-DISC1 or the different DISC1 variants (Left panel) or together with DN-LEF (Right panel). Cells were fixed, staining with DAPI, and the number of cells were quantified 48 hours later and represented as a combined graph. Quantification was performed by taking 4 representative images from each condition in each experiment (n=3 experiments, #,*p<0.05, **p<0.005, mean ± SEM). See also Figure S1.