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. Author manuscript; available in PMC: 2012 Jul 2.
Published in final edited form as: Biochemistry. 2011 Nov 8;50(47):10300–10310. doi: 10.1021/bi2009147

Figure 5.

Figure 5

Hsp70 does not rescue anterograde FAT when incubated with PAD peptide. Vesicle motility assays in isolated squid axoplasm. Individual velocity (micrometers per second) rate measurements (arrowheads) are plotted as a function of time (minutes). Black and red arrowheads and lines represent anterograde FAT rates (kinesin-dependent), and gray and green arrowheads and lines represent retrograde FAT rates (cytoplasmic dynein-dependent). (A) Perfusion of Hsp70 (1.6 μM) alone in axoplasm has a mild inhibitory effect on both anterograde and retrograde FAT. (B) Perfusion of PAD peptide (2 μM) results in a select inhibition of kinesin-dependent FAT as previously reported.15 (C) Co-incubation of Hsp70 (1.6 μM) with PAD peptide does not alter the inhibitory effect of PAD on anterograde FAT [p = 0.72 (unpaired t test)]. For comparison, dashed lines represent Hsp70 alone as shown in panel A; note that the green and gray lines representing retrograde transport are coincident in panel C.