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Verification of N-terminal fusions of recombinant MBP-MT3 and MBP-MT4. A, PageBlue protein stains of recombinant MBP-MT3 and MBP-MT4 proteins separated by SDS-PAGE. B and C, Immunoblotting using antibodies specific for barley MT3 (B) and MT4 (C). Purified MBP-MT (5 µg) was used on the immunoblots. The calculated sizes of MBP-MT3 and MBP-MT4 are 48.9 and 49.7 kD, respectively. MBP fusion proteins were consistently found to migrate approximately 8 kD higher than the calculated mass.
