Figure 1.

PavSSK1 was a single-copy gene and expressed in several floral organs. A, Genomic DNA-blot analysis using the PavSSK1 probe. Genomic DNA from sweet cherry cv Satonishiki was digested with DraI, EcoRI, or HindIII and probed with the PavSSK1 probe. B, PavSSK1 and PavPSK1 expression patterns were examined by RT-PCR. Total RNAs from various organs were used as templates for cDNA synthesis and RT-PCR with gene-specific primer pairs. L, Leaves; Sp, sepals; Pt, petals; Ov, ovaries; St, styles; Fl, filaments; A, anthers; P, pollen.