Figure 4.

Y2H analysis to investigate interactions between PavSSK1 and Prunus F-box proteins. A, A colony of AH109 cotransformants was grown on nonselective (-LW) and selective (-AHLW) media for 7 d at 25°C. The empty pAD-GAL4-2.1 vector (WT) and the empty pGBKT7 vector (Negative) were used as negative controls. B, β-Galactosidase activity was detected in a liquid assay using o-nitrophenyl-β-d-galactopyranoside as a substrate. The activity is given as the mean ± sd of five independent transformants.