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. 2012 May 1;159(3):1252–1262. doi: 10.1104/pp.112.197343

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Figure 6. — In vitro binding assays of PavSSK1-PavSFB and PavSSK1-PavCul1s. A, The interaction between PavSSK1 and PavSFB was tested. Purified GST-tagged PavSSK1 or GST-tagged PavSSK1ΔH0 was used as bait against crude cell-free expressed cMyc-SFB-S³ or cMyc-SFB-S⁶. Bound fractions were treated with thrombin before SDS-PAGE to dissociate the GST tag from the GST-fusion proteins. Bound proteins were detected using the anticMyc antibody. GST was used as a negative control. Triangles and asterisks indicate the GST-fusion proteins and the GST fragment, respectively. Left section: assays using cMyc-SFB-S³; right section: assays using cMyc-SFB-S⁶. B, The interaction between PavSSK1 and PavCul1s was tested. Purified GST-tagged PavSSK1 or GST-tagged PavSSK1ΔH0 was used as bait against crude cell-free expressed PavCul1A-3xHA or PavCul1B-3xHA. Bound proteins were detected using the anti-HA antibody. GST was used as a negative control. Triangles and asterisks indicate the GST-fusion protein and the GST fragment, respectively.