Table II. Selected proteins of the P. syringae DC3000 infectome in leaf crude extracts and CPLL-treated extracts.
Arabidopsis plants were infected with P. syringae DC3000, and extracted leaf proteins were analyzed directly by LC-MS/MS or subjected to CPLL before MS analyses. MW, Molecular mass in kD.
| Function | Accession No. | MW | No. of Peptides | Hints to Involvement in Virulence |
|---|---|---|---|---|
| Proteins identified in CPLL sample only | ||||
| Glc-1-P thymidylyltransferase | NP_790913 | 33 | 4 | Guo et al. (2012) |
| Gln synthetase | NP_795041 | 51 | 2 | Si et al. (2009) |
| Transcriptional regulator, LysR family | NP_795132 | 33 | 2 | Wharam et al. (1995) |
| RNA methyltransferase, TrmH family, group 3 | NP_794667 | 27 | 4 | Garbom et al. (2004) |
| Thr synthase | NP_791307 | 52 | 4 | Guo et al. (2012) |
| DNA topoisomerase I | NP_793294 | 97 | 2 | McNairn et al. (1995) |
| Amidophosphoribosyltransferase | NP_793585 | 56 | 3 | Guo et al. (2012) |
| Phosphate transport system protein PhoU | NP_795207 | 29 | 3 | Lamarche et al. (2008) |
| Ribose ABC transporter protein | NP_792188 | 15 | 2 | Kemner et al. (1997) |
| Acetyl-CoA carboxylase, biotin carboxylase | NP_794595 | 49 | 5 | Kurth et al. (2009) |
| ATP-dependent Clp protease, ATP-binding subunit ClpX | NP_793499 | 47 | 4 | Ibrahim et al. (2005) |
| Lysyl-tRNA synthetase | NP_791326 | 57 | 3 | Navarre et al. (2010) |
| Phosphoenolpyruvate carboxykinase | NP_790090 | 56 | 2 | Liu et al. (2005) |
| Argininosuccinate synthase | NP_793916 | 45 | 4 | Ardales et al. (2009) |
| Coronamic acid synthetase CmaB | NP_794454 | 35 | 2 | Buell et al. (2003) |
| dTDP-Glc 4,6-dehydratase | NP_790915 | 40 | 3 | Sen et al. (2011) |
| Proteins identified in both crude extract and CPLL sample | ||||
| Coronafacic acid synthetase, dehydratase component | NP_794431 | 18 | 6/4 | Buell et al. (2003) |
| Coronafacic acid synthetase, ligase component | NP_794429 | 55 | 4/12 | Buell et al. (2003) |
| GDP-Man 6-dehydrogenase AlgD | NP_791073 | 48 | 9/11 | Buell et al. (2003) |
| Catalase/peroxidase HPI | NP_794283 | 83 | 8/2 | Jittawuttipoka et al. (2009) |
| Hfq protein | NP_794675 | 9 | 3/3 | Schiano et al. (2010) |
| Translation elongation factor P | NP_791590 | 21 | 4/2 | Navarre et al. (2010) |
| ATP-dependent Clp protease, proteolytic subunit ClpP | NP_793500 | 24 | 5/3 | Ibrahim et al. (2005) |
| Secreted protein Hcp | NP_795162 | 19 | 8/2 | Mougous et al. (2006) |
| Proteins identified in crude extract only | ||||
| Alginate biosynthesis protein AlgF | NP_791063 | 23 | 6 | Buell et al. (2003) |
| Alginate lyase | NP_791066 | 42 | 2 | Buell et al. (2003) |
| Catalase | NP_794994 | 79 | 3 | Fones and Preston (2012) |
| Flagellin | NP_791772 | 29 | 2 | Buell et al. (2003) |
| Iron ABC transporter, periplasmic iron-binding protein | NP_790164 | 37 | 3 | Rodriguez and Smith (2006) |
| Levansucrase | NP_791279 | 48 | 2 | Li et al. (2006) |
| Outer membrane porin OprF | NP_792118 | 37 | 6 | Fito-Boncompte et al. (2011) |
| Outer membrane protein OmpH, putative | NP_791368 | 19 | 5 | Fito-Boncompte et al. (2011) |
| Periplasmic glucan biosynthesis protein | NP_794893 | 71 | 5 | Buell et al. (2003) |
| Phosphate ABC transporter | NP_793052 | 37 | 8 | Buell et al. (2003) |
| Protein-export protein SecB | NP_795055 | 18 | 5 | Hueck (1998) |
| Sugar ABC transporter, periplasmic sugar-binding protein | NP_790728 | 46 | 8 | Buell et al. (2003) |
| Superoxide dismutase, iron | NP_794118 | 21 | 3 | Fones and Preston (2012) |
| Thiol:disulfide interchange protein DsbA | NP_790191 | 23 | 7 | Ha et al. (2003) |
| Transcriptional regulator AlgQ | NP_789993 | 18 | 2 | Buell et al. (2003) |