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. 2012 May 10;159(3):961–974. doi: 10.1104/pp.112.197525

Figure 5.

Figure 5.

Protein expression patterns in pale-green mutants. A, RH3, ClpR2, and cpHSP70 protein levels in rh3-4, clpr2-1, and rh3-4/clpr2-1 mutants. Total leaf proteins (30 μg of protein or dilutions as indicated) from seedlings at leaf stage 1.07 were analyzed on immunoblots by probing with anti-RH3, anti-ClpR2, and anti-cpHSP70 antisera. The Ponceau S-stained membrane at the bottom shows sample loading and abundance. LHCP, Light-harvesting chlorophyll a/b-binding protein; RBCL, large subunit of Rubisco; WT, wild type. B, Immunoblot analysis of subunits of chloroplast protein complexes in rh3-4 and clpr2-1 mutants. Total leaf extracts (20 μg of protein or dilutions as indicated) from seedlings shown at the top at leaf stage 1.07 planted on one-half-strength Murashige and Skoog medium with 2% Suc were analyzed by immunoblotting probing with antibodies for the proteins named at the right. The blot stained with Ponceau S illustrates sample loading and the abundance of the large subunits of Rubisco.