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. Author manuscript; available in PMC: 2012 Jul 2.
Published in final edited form as: Methods Mol Biol. 2011;787:93–103. doi: 10.1007/978-1-61779-295-3_8

Fig. 1.

Fig. 1

Hsp40/Hsp70-dependent ubiquitination of GST–NBD1–R. (a) Efficient ubiquitination of GST–NBD1–R is only achieved in the presence of the Hsp40 and Hsp70 chaperone pair. All reactions contain E1 enzyme and E2 (UbcH5a) while the reaction in lane 1 is lacking the E3 CHIP as a negative control. (b) Time course of ubiquitination. A time-dependent increase in ubiquitination can be observed over the course of 3 h. Lane 1 represents the negative control in which CHIP and UbcH5a are not added to the reaction mixture.