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. Author manuscript; available in PMC: 2012 Jul 2.
Published in final edited form as: Mol Endocrinol. 1997 Dec;11(13):2016–2024. doi: 10.1210/mend.11.13.0042

Fig. 1. The CRE-Binding Activity Is Present in Extracts from the Anterior Hypothalamus of the Developing Rat.

Fig. 1

Gel shift analysis of a radioactively labeled oligonucleotide containing the CRE consensus sequence that was preincubated with increasing concentrations of protein extract (1.25, 2.5, 5, 10, and 20 μg) from the anterior hypothalamus of 12-to 13-day-old rats. No shift of the DNA fragment was detectable in the absence of brain extract (lane 1).