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. Author manuscript; available in PMC: 2012 Jul 2.
Published in final edited form as: Mol Endocrinol. 1997 Dec;11(13):2016–2024. doi: 10.1210/mend.11.13.0042

Fig. 3. CREB and Phosphorylated CREB Are Present in Both Anterior Hypothalamic and Cortical Extracts of the Developing Rat.

Fig. 3

Western blot analysis of 10 μg protein extract from the anterior hypothalamus (lanes 1 and 3) and cerebral cortex (lanes 2 and 4) of 12- to 13-day-old rats using antisera directed against CREB-1 (lanes 1 and 2) and phosphorylated CREB (lanes 3 and 4). A predominant, 43-kDa immunolabeled band is evident in all lanes, consistent with CREB. CREB and the phosphorylated form of CREB are present in the anterior hypothalamus (lanes 1 and 3) and in the cerebral cortex (lanes 2 and 4).