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. Author manuscript; available in PMC: 2012 Jul 2.
Published in final edited form as: Mol Endocrinol. 1997 Dec;11(13):2016–2024. doi: 10.1210/mend.11.13.0042

Fig. 7. Stress Increases the CRE-Binding Activity in the Anterior Hypothalamus but Not the Cerebral Cortex.

Fig. 7

Gel shift analysis of DNA containing the CRE consensus sequence using 10 μg protein extracts from the anterior hypothalamus (lanes 1–5) and the cerebral cortex (lanes 6–10). Lanes represent protein extracts from 12- to 13-day-old rats under stress-free conditions (lanes 1 and 6) or 0 min (lanes 2 and 7), 15 min (lanes 3 and 8), 30 min (lanes 4 and 9), and 60 min (lanes 5 and 10) after maximal hypothermic stress. Increased intensity of the CRE-binding activity signal is evident in the hypothalamic extracts after stress (lanes 3–5) as compared with the stress-free condition (lane 1). Signal intensity of the CRE- binding activity of cortical extracts is not consistently influenced by exposure to stress (lanes 7–10 compared with lane 6).