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. Author manuscript; available in PMC: 2012 Jul 2.
Published in final edited form as: Mol Endocrinol. 1997 Dec;11(13):2016–2024. doi: 10.1210/mend.11.13.0042

Fig. 9. Stress-Induced CRE-Binding Activity in the Hypothalamus Is Specific.

Fig. 9

A, Southwestern blot analysis of protein extracts from the anterior hypothalamus. Protein-bound membranes were incubated with 32P-labeled double-stranded oligonucleotide containing the CRE consensus sequence. Protein extracts obtained from 12- to 13-day-old rats exposed to stress shows reveal increased signal intensity compared with stress-free control. Size of resultant bands are consistent with CREB (43 kDa). B, Western blot analysis of same membrane using an antiserum directed against CREB-1. No increase in CREB immunoreactivity in extracts from animals exposed to stress is apparent.