Figure 3. LCI allows direct quantification of the modulation of NICD/RBPjk interactions in live cells.
A. Wild-type (but not WFP>LAA-mutant) RAM polypeptides reduced NΔE-NLuc/CLuc-RBPjκ complementation in a dose-dependent manner. G. MAML-EGFP and dnMAML-EGFP stabilize the interactions between the Notch RAM mutants and RBPjκ. * p<0.01, ** p<0.0001.