Figure 4. Notch-NLuc/CLuc-RBPjκ complementation occurs in the nucleus.

NΔE-NLuc/CLuc-RBPjκ complementation occurs in the nucleus as demonstrated by subcellular fractionation (A) and cellular bioluminescence imaging (B). This is in contrast to full-length luciferase, which partitions almost equally between the cytoplasmic and nuclear fractions (A) and can be found throughout the cell (B). Cellular bioluminescence images were processed using ImageJ to optimize the brightness and contrast and to reduce the salt-and-pepper noise in the background (i.e., despeckle).