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. Author manuscript; available in PMC: 2012 Jul 2.
Published in final edited form as: Methods Mol Biol. 2012;821:295–303. doi: 10.1007/978-1-61779-430-8_18

Fig. 3.

Fig. 3

Coadministration of BCG vaccine and rapamycin induces a better protection against tuberculosis correlating with increased CD8 T-cell responses. C57Bl/6 mice were vaccinated with BCG alone or BCG vaccine followed by 30 daily injections of rapamycin given i.p. On day 32, inguinal lymph nodes and spleens were analyzed for antigen-specific Th1 T-cell responses and then aerosol challenged with M. tuberculosis to determine protection. (a) On day 32, lymphoid compartments show an increased frequency of CD8 T cells that secrete IFNγ as analyzed by fl ow cytometry (four mice per group, t test). (b) Rapamycin combination with BCG vaccine enhances protection against tuberculosis compared to BCG vaccine alone (five mice per group; two separate experiments, p-values shown for groups compared, ANOVA).