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. Author manuscript; available in PMC: 2013 May 25.

Published in final edited form as: Circ Res. 2012 Apr 24;110(11):1435–1444. doi: 10.1161/CIRCRESAHA.112.269506

[A] The whole cell transmembrane current values of the thapsigargin-evoked current in PAECs treated with () and without () doxycycline are shown. Data were collected using the stepwise voltage protocol (see Material and Methods) in 130 mmol/L extracellular Na⁺ and various extracellular calcium concentrations ([Ca²⁺]_ext). Cells were clamped with the pipette containing 100 nmol/L free Ca²⁺ and 1 μmol/L thapsigargin, 130 mmol/L extracellular Na⁺ and the indicated [Ca²⁺]_ext. [B] Reversal potential of the thapsigargin-evoked current in PAECs treated with (closed bar) and without (open bar) doxycycline in the indicated [Ca²⁺]_ext is shown. Data were calculated using a piecewise cubic spline function. [C] The fraction decay of the thapsigargin-evoked whole cell current in PAECs treated with () and without () doxycycline, measured for 100 seconds at −80 mV using the stepwise voltage protocol. * P < 0.05 (doxycycline-treated vs. doxycycline non-treated, Student t-test.

Inline graphic — [A] The whole cell transmembrane current values of the thapsigargin-evoked current in PAECs treated with () and without () doxycycline are shown. Data were collected using the stepwise voltage protocol (see Material and Methods) in 130 mmol/L extracellular Na⁺ and various extracellular calcium concentrations ([Ca²⁺]_ext). Cells were clamped with the pipette containing 100 nmol/L free Ca²⁺ and 1 μmol/L thapsigargin, 130 mmol/L extracellular Na⁺ and the indicated [Ca²⁺]_ext. [B] Reversal potential of the thapsigargin-evoked current in PAECs treated with (closed bar) and without (open bar) doxycycline in the indicated [Ca²⁺]_ext is shown. Data were calculated using a piecewise cubic spline function. [C] The fraction decay of the thapsigargin-evoked whole cell current in PAECs treated with () and without () doxycycline, measured for 100 seconds at −80 mV using the stepwise voltage protocol. * P < 0.05 (doxycycline-treated vs. doxycycline non-treated, Student t-test.