Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2013 May 25.

Published in final edited form as: Circ Res. 2012 Apr 24;110(11):1435–1444. doi: 10.1161/CIRCRESAHA.112.269506

[A] Cells engineered for conditional regulation of Orai1 shRNA were grown to confluence in the presence or absence of doxycycline. In the absence of doxycycline, thapsigargin induced the formation of transient inter-endothelial cell gaps that resealed within 45 minutes. In contrast, gaps were not visible in cells lacking Orai1 following doxycycline treatment. Data are representative of 3 experiments. [B] Analysis of number of gaps (left panel) and the gap size (right panel) reveals thapsigargin induces multiple small gaps, ranging in size from 20-200 μm, with fewer gaps larger that 200 μm. Gaps were not visible in cells following Orai1 silencing. * P < 0.05 vs. time 0.

[A] Cells engineered for conditional regulation of Orai1 shRNA were grown to confluence in the presence or absence of doxycycline. In the absence of doxycycline, thapsigargin induced the formation of transient inter-endothelial cell gaps that resealed within 45 minutes. In contrast, gaps were not visible in cells lacking Orai1 following doxycycline treatment. Data are representative of 3 experiments. [B] Analysis of number of gaps (left panel) and the gap size (right panel) reveals thapsigargin induces multiple small gaps, ranging in size from 20-200 μm, with fewer gaps larger that 200 μm. Gaps were not visible in cells following Orai1 silencing. * P < 0.05 vs. time 0.