Table 1.
Lethal time 50 (LT50) for different fractions ofMedicago truncatulaseed flour onSitophilus oryzae
| Fractions | amount per g of food | LT50+/− SEM (day) |
|---|---|---|
|
M. truncatula seed flour |
10 mg |
> 20 |
| |
50 mg |
11.03 +/− 0.319 |
| |
100 mg |
7.61 +/− 0.28 |
| |
250 mg |
5.33 +/− 0.2 |
| After solvents extraction |
20 mg |
3.79 +/− 0.13 |
| Purified saponin |
20 μg |
not toxic |
| (3-GlcA-28-AraRhaxyl-medicagenate) |
100 μg |
16.57 +/− 1.89 |
| |
200 μg |
12.96 +/− 0.52 |
| |
400 μg |
9.59 +/− 0.39 |
| |
800 μg |
8.42 +/− 0.25 |
| |
1200 μg |
6.23 +/− 0.24 |
| 1600 μg | 5.63 +/− 0.28 |
The indicated amount of each powder was added to one gram of wheat flour. The food produced was given to weevils and mortality recorded every day for 20 days.