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. 2012 Jul 2;7(7):e40056. doi: 10.1371/journal.pone.0040056

Figure 7. Effect of glucocorticoid receptor (GR) on proinflammatory properties of 11β-HSD1 in J774A.1 macrophages.

Figure 7

A, J774A.1 macrophages were transfected with the expression vector for 11β-HSD1 (HSD1) or a corresponding empty vector (emp) using Lentivirus. Cells were treated with PA (100 µmol/L) or co-treated with glucocorticoid antagonist RU486 for 24 h. B, J774A.1 macrophages were activated by LPS(100 ng/ml) in the absence (con) or presence of increasing amounts of corticosterone (10−10 M to 10−6 M) for 24 h in steroid hormones free media. C, J774A.1 macrophages were activated by LPS (100 ng/ml) in the absence (con) or presence of increasing amounts of 11-dehydro corticosterone (10−10 M to 10−5 M) for 24 h in Charcoal Dextran Stripped Serum media. D, J774A.1 macrophages were transfected with the expression vector for 11β-HSD1 (HSD1) or a corresponding empty vector (emp) using Lentivirus. Cells were activated by LPS(100 ng/ml) in the absence (con) or presence of increasing amounts of 11-dehydro corticosterone (10−11 M to 10−5 M) for 24 h in Charcoal Dextran Stripped Serum media. mRNA for IL-6, MCP-1 and TNF-α were determined by real-time PCR. The results are shown as the means ± SEM of three individual experiments. *P<0.05; **P<0.01 vs emp+PA (A) or con (B–C) or emp (D), # P<0.05 vs HSD1+PA (A).