Skip to main content
. 2012 Jul 2;7(7):e39112. doi: 10.1371/journal.pone.0039112

Figure 4. Distribution of quinacrine in the brains of Mdr1 0/0 mice by fluorescence microscopy.

Figure 4

Mdr1 0/0 mice were treated with 20 mg/kg/day of quinacrine via IV tail-injection and euthanized after 1 h (A) and 3 h (B). In mice after 1 h, a high level of quinacrine fluorescence was seen in the nuclei of the cerebellum (CB) and hippocampus (HIP) as well as in the nerve cell bodies of the brainstem (BS), thalamus (THAL), and cortex (CTX). GC, granule cell layer; WM, white matter tract; DG, dentate gyrus; arrowheads, cell bodies of neurons or astrocytes. (B) In mice after 3 h, a high level of quinacrine fluorescence was observed only in the brainstem of quinacrine-treated mice (left) but not in the brainstem of PBS-treated mice (right). (C) Mice were treated with 40 mg/kg/day of quinacrine in a chocolate-flavored liquid diet for 25 days. A high level of quinacrine fluorescence was observed in the brainstem of quinacrine-treated mice (left), which was not observed in the brainstem of untreated mice (right). Scale bar in A represents 50 µm and applies to all images.