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. 2012 Jul 2;7(7):e39665. doi: 10.1371/journal.pone.0039665

Figure 5. Wortmannin inhibited the NFAT5 activation by hypertonicity and hypoxia in HEK293 cells.

Figure 5

A. Cultures at 300 mosmol were incubated with DMSO (D) or Wortmannin (W) by 1 hour. Then the cells were cultured in normoxia (N) or 8 hrs of hypoxia (H) and NFAT5 abundance was studied by Western blot. B. Cells cultured at 300 mosmol were transfected with HRE-Luciferase and 24 hrs after transfection the cells were incubated with DMSO (D) or Wortmannin (W) by 1 hour. After this treatment, the cells were cultured in normoxia (300 or 500 mOsM) or 8 hrs of hypoxia (300 or 500 mOsM) and the luciferase activity was assayed. C. Cells cultured at 300 mosmol were cotransfected with HRE-Luciferase and siRNA (control or NFAT5). 48 hrs after transfection the cells were cultured in normoxia or 8 hrs of hypoxia (300 or 500 mOsM) and the luciferase activity was assayed. Bar graph represents Mean ± SEM. *, P<0.05; n = 5.