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. 2012 Jul 2;7(7):e39888. doi: 10.1371/journal.pone.0039888

Figure 7. P. aeruginosa flagellin induces MUC5AC expression through IL-8 signalling.

Figure 7

(A) NCI-H292 cells were exposed to PAK- or ΔFliC bacteria supernatant for 3, 6, 10 or 24 hrs and the IL-8 secreted into culture supernatants was measured using an ELISA, as described in Materials and Methods. (B) Shows IL-8 secretion by NCI-H292 cells after transfection by control siRNA (C), Naip siRNA or TLR5 siRNA with or without stimulation with flagellin (at 1 µg/ml). (C) Shows MUC5AC expression by NCI-H292 cells after incubation with flagellin with anti-IL-8 neutralizing or isotype control antibody. (D) Shows Induction of MUC5AC mRNA expression in NCI-H292 cells exposed to a rIL-8 (at 10 ng/ml). (E, F) shows the effect of ERK1/2 inhibitor PD98059 (20 µM) on MUC5AC expression by NCI-H292 cells stimulated with PAK supernatant or with purified flagellin. Values represent means ± SM of three independent experiments. * P<0.05, ** P<0.01, *** P<0.001 vs. LB.