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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Mol Cancer Res. 2012 Apr 18;10(6):834–844. doi: 10.1158/1541-7786.MCR-12-0025

Figure 1.

Figure 1

Aurora-A interacts with FBXW7 and GSK3β. (A and B) Coimmunoprecipitation of Flag-tagged Aurora-A and HA-tagged FBXW7 in transiently transfected 293T cells. Immunoprecipitations were performed with either (A) anti-Flag or (B) anti-HA antibodies. Immunoprecipitates were fractionated by SDS-PAGE and subsequently immunoblotted with (A) anti-HA or (B) anti-Flag antibodies. Coimmunoprecipitation of endogenous GSK3β was also detected using anti-GSK3β antibody. Lower panel shows the input levels of FBXW7, GSK3β and Aurora-A in the lysates. (C) Coimmunoprecipitation of HA-tagged FBXW7 with endogenous Aurora-A. 293T cells were transiently transfected with HA-tagged FBXW7. Immunoprecipitation was performed with anti-HA antibody and subsequently immunoblotted with anti-Aurora-A antibody. Lower panel shows the input levels of FBXW7 and Aurora-A in the lysates. All results are representative of three independent experiments.