Figure 7.

CD11c⁺ cell-induced early IL-13 secretion is important for disease resolution by PCN-dosed mice. A: Mice are dosed five times daily with PCN or PBS a week before MRSA infection and sacrificed 24 hours after infection. The bacterial burden in lungs is determined. B: PCN- and PBS-dosed mice are infected with 1 × 10⁸ CFUs of MRSA. FACS staining is performed on cells isolated from the BALF of these mice 2.5 hours after MRSA infection. Representative plots of IL-13⁺ CD11c⁺ cells are shown compared with these CD11c⁺ cells stained with isotype control Abs. C and D: Mice are depleted of CD11c⁺ cells on day −3, −1, and 0, in relation to MRSA infection, as described in the legend for Figure 6C. Mice are sacrificed 4 hours after infection, and the concentration of IL-13 (C) and IL-4 (D) is determined in their cell-free BALF. Each experiment is performed twice. The mean ± SD of three to four mice per group from a single experiment is shown. **P < 0.01, ***P < 0.001 versus PCN-dosed mice.