Figure 6.

IFrag^−/− mice develop a deviated bone marrow cytokine response promoting osteoclastogenesis, suggesting an unbalanced RANKL/OPG/TRAIL axis. Cytokine protein analysis was performed from bone marrow cell lysates at day 0, 7, and 10 after Pneumocystis lung infection using an ELISA-based multiplex technique for M-CSF (A), IL-6 (B), IL-1α (C), IL-1β (D), TNF-α (E), RANKL (F), OPG (G), and TRAIL (H). Results demonstrate a deviated cytokine response in bone marrow from IFrag^−/− mice consistent with osteoclastogenesis and bone marrow cell apoptosis. Statistical analysis was performed using a two-way analysis of variance. Values shown are mean ± SEM (n = 4). *P < 0.05, **P < 0.01, and ***P < 0.001.