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Peritoneal macrophage culture under starvation. Macrophages were collected from the peritoneal space and cultured using serum-free medium. A: WT mice show cytosolic (normal) and nuclear (apoptotic) staining for AIF, whereas AIF-deficient mice show no staining. B: TUNEL+ apoptotic macrophages increase after 24 hours of starvation in WT mice. In contrast, macrophages from AIF-deficient mice are resistant to starvation. *P < 0.05. Hq/Y, harlequin hemizygous male mice.
