Figure 1.

CLIC4^NULL mice develop spontaneous skin erosions. A: Protein lysates were isolated from spleen, heart, testis, lungs, liver, kidney, skeletal muscle, and skin of WT and CLIC4^NULL mice and were probed for CLIC4, CLIC1, β-actin, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) expression. B: Mean ± SEM litter size of WT and CLIC4^NULL matings. (WT: 8.31 ± 0.33, n = 32; CLIC4^NULL: 5.32 ± 0.42, n = 28; *P ≤ 0.0001). C: A comparison of the overall size of WT, CLIC4^HET, and CLIC4^NULL pups from one litter; approximately 10% of CLIC4^NULL pups are visibly smaller. D–F: Skin erosions in CLIC4^NULL mice in CD1 background (D) and C57/BL6 background (E and F).