Figure 3.

The loss of CLIC4 delays wound healing. An 8-mm, circular, full-thickness wound was made on the backs of eight WT and eight CLIC4^NULL mice, and the mice were subsequently monitored for 12 days. After 8 days, four independent WT mice (A–D) and four independent CLIC4^NULL mice (E–H) were photographed. I–P: The same mice were photographed after 12 days to monitor the extent of wound closure. Skin from the back wound site of four independent WT (Q–T) and CLIC4^NULL (U–X) mice was collected after 12 days, fixed in 10% neutral buffered formalin, and H&E stained. Sections show the epidermis and dermis at the wound site. Scale bar = 200 μm (Q–X).