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. 2012 Jul;181(1):74–84. doi: 10.1016/j.ajpath.2012.03.025

Figure 4.

Figure 4

Delayed resolution of immune cells in wounds in CLIC4NULL mice. IHC staining was performed on 2-, 4-, and 12-day-old full-thickness wounds using antibodies to detect inflammatory infiltrates (CD45; A–F) and macrophages (F4/80; G–L). Sections show the dermis of the wound site on a WT mouse stained for CD45 (A–C) and F4/80 (G–I) and on a CLIC4NULL mouse stained for CD45 (D–F) and F4/80 (J–L). Scale bars: 50 μm. M: Mean ± SEM CD45-positive cells in 400 μm of the wound area were counted by Aperio Imagescope software version 11.0.2.725: WT (n = 4), 152 ± 65; CLIC4NULL (n = 3), 346 ± 107. N: Mean ± SEM F4/80-positive cells in 250 μm of wound area: WT (n = 4), 97 ± 4; CLIC4NULL (n = 3), 147 ± 20. *P = 0.03.