Figure 7.

Attachment and cell cycle proteins differ in WT and CLIC4^NULL skin. A: Protein samples were isolated from untreated resting skin of six independent WT and six CLIC4^NULL mice. Samples were immunoblotted for β-actin, CLIC4, CLIC1, c-Myc, p21, β1 integrin, β4 integrin, α3 integrin, and E-cadherin. B and C: Densitometry on X-ray films was performed using ImageJ software to quantify levels of p21 (B) and β4 integrin (C). Expression levels are normalized to their respective β-actin controls and are presented as the mean ± SEM of six values: p21: WT, 6070 ± 1066; CLIC4^NULL, 2891 ± 490; β4 integrin: WT, 9753 ± 594; CLIC4^NULL, 5318 ± 1123. *P = 0.022, **P = 0.0058. D: Protein isolates from wound sites 4 days after full-thickness wounds from two independent WT and CLIC4^NULL mice were used for analysis of β-actin, β4 integrin, p21, and E-cadherin E: Protein isolates from unwounded and wounded corneal samples taken 18 hours after superficial debridement wounds were used for immunoblotting to detect β-actin, β4 integrin, p21, and E-cadherin.