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Ectopic expression of wild-type (WT) EGFR, activated EGFR mutants, and the K-rasV12 mutant in rat lung epithelial cells (RL-65) induces Fn14 expression. Total cellular lysates from RL-65 cells stably expressing the indicated EGFR receptors or K-rasV12 were prepared and analyzed by immunoblotting for Fn14, p-EGFR, and total EGFR. GAPDH was used as a loading control.
