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. 2012 Jul;181(1):111–120. doi: 10.1016/j.ajpath.2012.03.026

Figure 6.

Figure 6

Depletion of Fn14 reduces EGF-stimulated NSCLC cell migration and invasion. A: Serum-starved A549 cells were treated with 50 ng/mL EGF for the indicated times. Cells were harvested, and total cell lysates were prepared and immunoblotted with an antibody against Fn14. GAPDH was used as a loading control. B: A549 cells were infected with lentivirus expressing an Fn14 shRNA (156) or control nontargeting shRNA. Stable cell lines were isolated, cell lysates were prepared, and immunoblotting was conducted using the indicated antibodies against Fn14 and GAPDH (loading control). C: Transwell migration assay of A549 cells expressing control or Fn14 shRNA and either left untreated or treated with 50 ng/mL EGF. D: Matrigel invasion assay of A549 cells expressing control or Fn14 shRNA and either left untreated or treated with 50 ng/mL EGF. For migration and invasion assays, the values shown are the mean ± SEM of triplicate chambers. Significance was assessed by the Student's t-test. **P < 0.01.