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. 2012 Jul;181(1):111–120. doi: 10.1016/j.ajpath.2012.03.026

Figure 7.

Figure 7

Fn14 overexpression enhances NSCLC cell migration and invasion in vitro. A: A549 cells were infected with lentivirus expressing full-length hFn14-HA or vector alone (control). Stable cell lines were isolated, cell lysates were prepared, and immunoblotting was conducted using antibodies against HA and tubulin (loading control). B: Transwell migration assay of control and Fn14-overexpressing A549 cells. C: Matrigel invasion assay of control and Fn14-overexpressing A549 cells. For migration and invasion assays, the values shown are the mean ± SEM of triplicate chambers. Significance was assessed by the Student's t-test. **P < 0.01.