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The S20G-IAPP peptide is toxic to cultured rat INS-1 β-cells. Cell viability was determined by Alamar Blue reduction assays. Rat IAPP was used as negative control. All peptide solutions were incubated for 4 hrs at room temperature in 20mM Tris-HCl buffer at pH 7.4 and then applied to rat INS-1 β-cells in 96-well plates. INS-1 β-cells were stimulated with IAPP solution for 5 hours prior to cell viability measurement.
