Skip to main content
. 2012 May 19;3(5):535–545. doi: 10.18632/oncotarget.498

Figure 2. Validation of compounds identified in the primary screen.

Figure 2

GIMEN (MYCN non-amplified neuroblastoma cells) were transiently co-transfected with the MYB-responsive promoter pGL2-mim1 and the pcDna-B-MYB/empty vectors or with the p53 responsive p21luc promoter and pCMVp53/empty vectors. A renilla luciferase plasmid was added to the transfection mix for normalisation. Cells were treated with the compounds ellipticine, pinafide and camptothecin at the indicated concentrations for 24h. Error bars indicate standard deviations and the asterisk indicates statistically significant differences (Student's T test p ≤ 0,05) between the activities of cells treated with the compound relative to untreated cells (indicated by ctrl).